• Blasticidin S packaged and labeled in glass vial.

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SKU: B052

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Description

Blasticidin S is a peptidyl nucleoside produced by several species of Streptomyces that was first isolated from S. griseochromogenes in 1958.  Blasticidin S inhibits protein synthesis and is active against bacteria, fungi, nematodes, and tumor cells.  The compound is used as a selection antibiotic for both eukaryotic and prokaryotic cells, and a marker for strain manipulation.

 TOKU-E carries three forms of Blasticidin S:

 Blasticidin S is soluble in water, methanol, DMF or DMSO.

    CAS Number

    2079-00-7

    Molecular Formula

    C17H26N8O5

    Molecular Weight

    422.4

    Mechanism of Action

    Blasticidin S inhibits protein synthesis in prokaryotic and eukaryotic cells by binding to the ribosomal P-site which strengthens tRNA binding and slows down and prevents subsequent peptide synthesis.

    Mechanisms of resistance

    Resistance to Blasticidin S is conferred by bsr, BSD, and bls resistance genes isolated from Bacillus cereus K55-S1, Aspergillus terreus, and Streptoverticillum spp, respectively.

    The bsr resistance gene is a 420 bp fragment and encodes a 15 kDa Blasticidin S deaminase which catalyzes the reaction of Blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of Blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes.

    The bsd resistance gene is a 393 bp fragment and also encodes a Blasticidin S deaminase enzyme which catalyzes a similar reaction to the BSR deaminase. A study by Kimura et al. found the transfection frequency with bsd to be 80X greater than with bsr when using FM3A cells.

    The bls gene resistance gene encodes an acetyltransferase which interacts with acetyl-coenzyme A and prevents Blasticidin S from inhibiting protein synthesis. 

    Storage Conditions

    -20°C

    Spectrum

    Blasticidin S is active against mammalian and prokaryotic cells.

Applications

    Eukaryotic Cell Culture Applications

    Blasticidin is selection antibiotic for both eukaryotic and prokaryotic cells. Resistance to Blasticidin is conferred by the following genes:

    1) Bsr (blasticidin resistance), from Bacillus aureus, which codes for Blasticidin-S deaminase.

    2) Bls (blasticidin S acetyltransferase), from Streptoverticillum spp.

    3) BSD (blasticidin S deaminase), from Apergillus terreus.

    Typically, mammalian cells are sensitive to concentrations of 1-10 µg/ml, and bacteria to 25-100 µg/ml. Blasticidin can be used for systematic screening of cytokinesis mutants using an improved restriction enzyme-mediated integration system (REMI) in mutant Dictyostelium cells.

    Microbiology Applications

    Blasticidin S can be used as a selection agent after transformation of prokaryotic cells such as E. coli.

    Plant Biology Applications

    Blasticidin S is an antifungal agent with particularly potent activity against the rice pathogen, Piricularia oryzae, for which it was used commercially in Japan.

Specifications

    Appearance

    White solid

References

    References

    Adachi H, Hasebe T, Yoshinaga K , Ohta T and Sutoh K (1994) Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the Blasticidin S resistance marker. Biochem. Biophys. Res. Comm. 205(3):1808-1814

    Bento, FM (2004) Over Expression of the Selectable Marker Blasticidin S Deaminase Gene Is Toxic to Human Keratinocytes and Murine BALB/MK Cells." BMC Biotechnol. 4 (29):1-10 PMID 15575952.

    Izumi M. et al., 1991. Blasticidin S-resistance gene (bsr): A novel selectable marker for mammalian cells. Exp.Cell Res.197:229-33

    Kimura M, Takatsuki A, Yamaguchi I (1994) Blasticidin S deaminase gene from Aspergillus terreus(BSD): A new drug resistance gene for transfection of mammalian cells. Biochim. Biophys. Acta. 1219(3):653-65 PMID 7948022

    Lu K-T et al (2016) Ovatodiolide inhibits breast cancer stem/progenitor cells through SMURF2-mediated downregulation of Hsp27. Toxins 8(5):127

    Svidritskiy E, Ling C, Ermolenko DN, Korostelev AA (2013) Blasticidin S Inhibits Translation by Trapping Deformed TRNA on the Ribosome. PNAS 110(30):12283-12288 PMID 23824292

    Takeuchi S, Hirayama K, Ueda K, Sakai H and Yonehara H (1958) Blasticidin S, a new antibiotic. J. Antibiot. 11(1):1-5 PMID 13525246

    Yamaguchi I et al (1990) Expression of the Blasticidin S Deaminase Gene (bsr) in Tobacco: Fungicide Tolerance and a New Selective Marker for Transgenic Plants. Mol. Gen. Genet (2):332-334 PMID 2250657

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