• Blasticidin S HCl, 10 mg/mL packaged and labeled.

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SKU: B006

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Description

Blasticidin S HCl is a nucleoside antibiotic derived from Streptomyces griseochromogenes. Blasticidin S HCl is routinely used in cell culture gene selection applications as a selection agent to isolate mammalian cells containing the blasticidin S-resistance (bsr) gene.

Blasticidin S HCl solution contains 10 mg/mL blasticidin S HCl in 20mM HEPES. 10 x 1 mL contains 10 mg blasticidin S HCl per vial (100 mg total). 20 mL contains 200 mg blasticidin S HCl.

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    CAS Number

    3513-03-9

    Molecular Formula

    C17H26N8O5 · HCl

    Molecular Weight

    458.90 g/mol

    Mechanism of Action

    Blasticidin S HCl inhibits protein synthesis in prokaryotic and eukaryotic cells by binding to the ribosomal P-site.. Blasticidin S HCl strengthens tRNA binding to the P-site which slows down and prevents subsequent peptide synthesis.

    Storage Conditions

    -20┬░C

    Tariff Code

    2934.99.9000

Applications

    Eukaryotic Cell Culture Applications

    Blasticidin S HCl is frequently used as a selection agent after transfection of eukaryotic cells. Selectable markers include blasticidin S deaminase resistant genes bsr and bsd.

    For more information on relevant cell lines, culture medium, and working concentrations, please visit the TOKU-E Cell-culture Database.

    Microbiology Applications

    Blasticidin S HCl can be used as a selection agent after transformation of prokaryotic (bacterial) cells.

Specifications

    Form

    Solution (sterile)

    Appearance

    Clear and colorless or light yellow solution

    Source

    Streptomyces griseochromogenes

    pH

    7.2-7.5

    Concentration

    10.0±0.3 mg/mL

References

    References

    Yamaguchi, I., et al. "Expression of the Blasticidin S Deaminase Gene (bsr) in Tobacco: Fungicide Tolerance and a New Selective Marker for Transgenic Plants." Molecular and General Genetics 223.2 (1990): 332-34. www.ncbi.gov. Web. 7 Sept. 2012.

    "Blasticidin S Inhibits Translation by Trapping Deformed TRNA on the Ribosome." PNAS 110.30 (2013): 12283-2288. Ncbi.nlm.nih.gov. Web. 4 Sept. 2014.

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