• Amphotericin B, EP packaged and labeled.

click on thumbnail to zoom
SKU: A064

Size  
Quantity:
  Click 'Bulk Order' for 10 or more
Price: make selection(s)
Description

Amphotericin B, EP is a polyene antifungal or antimycotic compound derived from Streptomyces nodosus. It is used to control contamination from fungi, viruses and protozoa.

TOKU-E offers 3 forms of Amphotericin B:

The compound is nearly insoluble in water at pH 6-7 (but is soluble at pH 2 or 11).  It is soluble in dimethyl sulfoxide and dimethylformamide. 

Amphotericin B, EP conforms to European Pharmacopoeia specifications.

    CAS Number

    1397-89-3

    Molecular Formula

    C47H73NO17

    Molecular Weight

    924.08

    Mechanism of Action

    Amphotericin B associates with membrane sterols (ergosterol in fungal cell membranes, and cholesterol in mammalian cell membranes). Amphotericin B forms a pore in these membranes resulting in leakage of essential ions and ultimately cell death.

    Storage Conditions

    2-8°C

    Tariff Code

    2941.90.1050

    Spectrum

    Amphotericin B is active against mammalian cells, fungi, viruses, and protozoa. Amphotericin B is not toxic to bacteria due to their lack of sterols..

    The following represents MIC susceptibility data for amphotericin B against common fungal pathogens:

    • Candida albicans - 0.001 - 321 μg/mL
    • Candida krusei - 0.001 - 16 μg/mL
    • Coccidioides immitis - 0.0625 - 2 μg/mL
    • Cryptococcus neoformans - 0.2 - 39 μg/mL
    • Fusarium oxysporum - 0.75 - 125 μg/mL

Applications

    Eukaryotic Cell Culture Applications

    Amphotericin B can be used in eukaryotic cell culture to control or prevent contamination from fungi, viruses, and protozoa. Amphotericin B can be toxic to cell lines at high concentrations and should not be used at concentrations greater than 2.50 µg/mL. Amphotericin B has been used in in vitro to inhibit the generation of the scrapie isoform of the prion protein when studying transmissible spongiform encephalopathies (Mangé et al 2000).

    Microbiology Applications

    Amphotericin B is used as an antimycotic selective agent in several routinely used selective media formulations to inhibit the growth of background fungal growth. It can also combat viruses and protozoa.

    Plant Biology Applications

    Amphotericin B can be used to inhibit phytopathogenic fungi in vitro

Specifications

    Form

    Powder

    Appearance

    Yellow or Orange hygroscopic powder

    Source

    Streptomyces nodosus

    Absorbance

    UV max: 362, 381 and 405nm
    UV ratios:
    A362/A381 = 0.57-0.61
    A381/A405 = 0.87-0.93

    Assay

    (On Dried Basis): ≥750 IU/mg

    Loss on Drying

    ≤5.0%

    Sulfated Ash

    ≤3.0%

    Impurities

    Related Substances
    Impurity A (303 nm): ≤5.0%
    Any Other Impurity (303 nm): ≤1.0%
    Impurity B (383 nm): ≤4.0%
    Any Other Impurity (383 nm): ≤2.0%
    Total Impurities (303 nm and 383 nm): ≤15.0%

References

    References

    Brajtburg, J, Powderly WG, Kobayashi GS, and Medoff G. (1990) Amphotericin B: Current understanding of mechanisms of action. Antimicrob. Agents and Chemother. 34 (2):183-88. PMID 2183713

    Mangé A et al. (2000) Amphotericin B inhibits the generation of the scrapie isoform of the prion protein in infected cultures. J. Virol. 74(7):3135-3140 PMID 10708429

    Perez-de-Luque A et al. (2012) Effect of Amphotericin B nanodisks on plant fungal diseases. Pest Manag. Sci. 68(1):67-74. PMID 21710554

    Rice, LB, and Ghannoum MA (1999). Antifungal Agents: Mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin. Microbiol. Rev. 12(4):501-517 PMID 10515900

    Radomski N, Cambau E, Moulin L, Haenn S, Moilleron R, and Lucas FS (2010) Comparison of culture methods for isolation of nontuberculous Mycobacteria from surfacewaters. Appl. Environ. Microbiol 76(11):3514-3520 PMID 20363776

    Schaffner CP et al (1986) Anti-viral activity of Amphotericin B methyl ester: inhibition of HTLV-III replication in cell culture. Biochem. Pharmacol. 35(22):4110-4113 PMID 3640625

    Sokol-Anderson ML, Braitburg J, Medoff G (1986) Amphotericin B-induced oxidative damage and killing of Candida albicans. J. Infect. Dis. 154(1):76-83 PMID 3519792

Other Items In This Category