Kanamycin sulfate, USP packaged and labeled.

Available Discounts

Microbiology promotion
  • buy any, save 20%
53.71142.64
SKUSizeQtyPrice
K0085 g
$53.71Add to Cart
K00825 g
$142.64Add to Cart
Bulk Order
  • Description

    Kanamycin, USP is an aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance.

    Standard grade kanamycin is composed of a mixture of three different fractions: kanamycin A, B, and C.  TOKU-E offers four forms of kanamycin:

    EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein. Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.

    Spectrum

    Kanamycin sulfate is a broad spectrum antibiotic but is most active against aerobic Gram-negative bacteria.

    • Microbiology Applications

      Kanamycin sulfate is commonly used as a selective agent to select for resistant mammalian, fungal, or bacterial cells that contain the kanMX marker or other kanamycin resistance genes. Kanamycin sulfate is typically used at a concentration of 50 µg/mL.

      Pryjma, et al. from the University of British Columbia used TOKU-E kanamycin sulfate to select for transformed kanamycin resistant Campylobacter jejuni cells: "FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection"

       

      Media Supplements

      Kanamycin can be used as a selective agent in several types of isolation media:

      Kanamycin Aesculin Azide AgarEnterococci isolation in food

      Perfringens Agar - SFP and TSC selective supplements for the isolation of Clostridium perfringens

    • Plant Biology Applications

      Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.

    • References

      Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

      United States. National Institutes of Health. Kanamycin Compound SummaryPubChem. Web. 21 Aug. 2012.

    Form

    Powder

    Purity Level

    ≥95.0%

    Tariff Code

    2941.90.1010

    Related Documents


    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to info@toku-e.com

  • CAS Number25389-94-0
    Molecular FormulaC18H36N4O11 · H2SO4
    Molecular Weight582.58 g/mol
    FormPowder
    AppearanceWhite to off-white crystalline powder
    Purity Level≥95.0%
    SolubilityWater: Freely soluble
    SourceStreptomyces Kanamyceticus.

    Storage ConditionsStock solutions should be stored at 2-8 °C.