Kanamycin acid sulfate packaged and labeled.

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  • Description

    Kanamycin is an aminoglycoside antibiotic often used to select for bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance.  Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.

    Standard grade kanamycin is composed of a mixture of three different fractions: kanamycin A, B, and C. TOKU-E offers five forms of kanamycin: kanamycin sulfate (K008), kanamycin acid sulphate (K004), kanamycin A sulfate, EvoPure® (K013), kanamycin B sulfate (K002), and kanamycin B sulfate, EvoPure® (K014). EvoPure® products are purified single antibiotic fractions, most >99% pure.  High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.

    Mechanism of Action

    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.

    Spectrum

    Kanamycin is a broad spectrum antibiotic; however, it is mostly used against aerobic gram negative bacteria.

    • Microbiology Applications

      Kanamycin acid sulfate is commonly used as a selective agent to select for resistant mammalian, fungal, or bacterial cells that contain the kanMX marker or other kanamycin resistance genes. Kanamycin acid sulfate is typically used at a concentration of 50 µg/mL.

      Pryjma, et al. from the University of British Columbia used TOKU-E kanamycin sulfate to select for transformed kanamycin resistant Campylobacter jejuni cells: "FdhTU-Modulated Formate Dehydrogenase Expression and Electron Donor Availability Enhance Recovery of Campylobacter jejuni following Host Cell Infection"

       

      Media Supplements

      Kanamycin can be used as a selective agent in several types of isolation media:

      Kanamycin Aesculin Azide AgarEnterococci isolation in food

      Perfringens Agar - SFP and TSC selective supplements for the isolation of Clostridium perfringens

    • Plant Biology Applications

      Kanamycin is often used in the Agrobacterium mediated transformation while using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.

    • References

      Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.

      United States. National Institutes of Health. Kanamycin Compound SummaryPubChem. Web. 21 Aug. 2012.

    Form

    Powder

    Tariff Code

    2941.90.1010

    Related Documents


    For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to info@toku-e.com

  • CAS Number64013-70-3
    Molecular FormulaC18H36N4O11 · 2 H2SO4
    Molecular Weight680.65 g/mol
    FormPowder
    AppearanceColorless solid
    SolubilityWater: Freely soluble
    SourceStreptomyces Kanamyceticus
    Potency (on a dry basis)670 IU/mg
    Storage Conditions2-8 °C