Kanamycin is an aminoglycoside antibiotic often used to select for plants (Agrobacterium mediated transformation) and bacteria which have been successfully transformed with a plasmid conferring kanamycin resistance. Kanamycin is very soluble in aqueous solution at 92.3 mg/mL.
Standard grade kanamycin is composed of a mixture of three different fractions: kanamycin A, B, and C. TOKU-E offers five forms of kanamycin:
EvoPure® products are purified single antibiotic fractions, most >99% pure. High purity EvoPure® kanamycin products can be used to analyze the specific effects of individual kanamycin fractions.
Mechanism of Action
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
Kanamycin is a broad spectrum antibiotic, however, it is mostly used against aerobic Gram-negative bacteria.
Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
- Salmonella enteritidis 62.5 µg/mL
- Escherichia coli 31.2 µg/mL
- For a complete list of kanamycin MIC values, click here.
Kanamycin can be used as a selective agent in several types of isolation media:
Kanamycin Aesculin Azide Agar - Enterococci isolation in food
Plant Biology Applications
Kanamycin is often used in Agrobacterium mediated transformation using the npt II gene as selection marker. Kaur and Bansal (2010) used kanamycin in combination with cefotaxime to control bacterial growth while transforming tomato.
Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.
Aragão F.J.L. and Brasileiro A.C.M., 2009 Positive, negative and marker-free strategies for transgenic plant selection. Braz. J. Plant Physiol., 14(1):1-10, 2002
Sharma M.K., Solanke A. U., Jani D., Singh Yogendra and Sharma A.K. A simple and efficient Agrobacterium-mediated procedure for transformation of tomato. J. Biosci. 34(3), September 2009, 423–433.Aragão F.J.L. and Brasileiro
Kaur P., and Bansal K.C. Efficient production of transgenic tomatoes via Agrobacterium-mediated transformation. Biologica plantarum 54 (2): 344-348, 2010
For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to [email protected]