Gentamicin A is one of several different components found in standard grade gentamicin. Gentamicin A, gentamicin B, gentamicin X2, and others are considered “minor components,” have little antimicrobial activity, and account for approximately 20% of standard grade gentamicin. The major components of gentamicin are members of the gentamicin C complex which account for up to 80% of standard grade gentamicin and possess higher antimicrobial properties. The minor gentamicin components (A, B, X2, etc..) contain hydroxy groups in the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) ring. The gentamicin C complex does not have hydroxy groups at these locations. This structural difference is thought to account for the difference in antimicrobial activity between the minor (gentamicins A, B, X2, etc..) and the gentamicin C complex. Gentamicin A sulfate, EvoPure® is ≥99.0% gentamicin A.
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Huang et al. used gentamicin A sulfate, EvoPure® and gentamicin X2 sulfate, EvoPure® to study enzymatic conversion of gentamicin A2 to other gentamicin congeners. "Delineating the Biosynthesis of Gentamicin X2, the Common Precursor of the Gentamicin C Antibiotic Complex."
Mechanism of Action
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
Gentamicin is a broad spectrum antibiotic targeting a wide range of Gram positive and Gram negative bacteria. Interestingly, gentamicin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
Gentamicin EvoPure® compounds can be used to study effects of individual gentamicin components on various bacterial strains.
Davis, Bernard D. "Mechanism of Bactericidal Action of Aminoglycosides."Microbiological Reviews 51.3 (1987): 341-50.
Sarna, Katarzyna, and Agata Blazewicz. "Determination of Gentamicin Sulphate Composition and Related Substances in Pharmaceutical Preparations by LC with Charged Aerosol Detection." PubMed (2010)Vydrin, A. F. "Component Composition of Gentamicin Sulfate Preparations." Pharmaceutical Chemistry Journal 37.8 (2003): 448-49. Web. 15 Oct. 2013.
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