Cycloheximide is a protein synthesis inhibitor in eukaryotic cells and is commonly used to prevent fungal growth.
TOKU-E offers three forms of cycloheximide: cycloheximide (C001), cycloheximide solution (C084), and cycloheximide ultrapure (C071). Cycloheximide solution is dissolved in DMSO at 100 mg/mL. Cycloheximide ultrapure is ≥98% pure and is free of toxic isomers that can potentially kill wanted fungi in a selection media.
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.
Mechanism of Action
Cycloheximide prevents ribosomal translocation during translation as shown in a yeast model. Effective doses are present at concentrations around 200 µM to inhibit yeast growth.
Plant Biology Applications
Cycloheximide is a frequently used lab reagent to inhibit fungal growth by targeting protein synthesis. In yeast, concentrations of 200 µM have fungicidal effects. (Schneider-Poetsch, 2010).
Doyle S.M., Diamond M. and McCabe P.F, 2009, Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, Vol. 61, No. 2, pp. 473–482, 2010
Schneider-Poetsch T., Ju J., Eyler D.E., Dang Y., Bhat S., Merrick W.C., Green R. and Shen B., 2009, Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nature chemical biology, vol 6, march 2010.
For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to [email protected]