Antibiotic-antimycotic solution is effective against both Gram-positive and Gram-negative bacteria as well as yeasts and other fungi in eukaryotic cell culture. The solution is composed of penicillin (10,000 units/mL), streptomycin (10,000 ug/mL), and amphotericin B (25 ug/mL) in 0.9% NaCl.
Naleway et al. used antibiotic-antimycotic solution from TOKU-E in RPMI 1640 media to grow Primary Human Fibroblasts. "Metabolic Lysosomal Enzyme Probes."
Mechanism of Action
The penicillin works by destroying the cell walls of non-resistant cells by preventing peptidoglycan linkage followed by the triggering of autolysis. Streptomycin disrupts bacterial protein synthesis by binding the ribosomes. Amphotericin B, the anti-fungal element, works by increasing the permeability of the fungal cell membrane by binding to ergosterol .
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