Vancomycin HCl, USP is a bactericidal glycopeptide antibiotic.
Vancomycin HCl, USP is sparingly soluble in aqueous solution (0.225 mg/mL) and >80.0% pure. EvoPure® products are purified single antibiotic fractions, most >99% pure. Click here for more vancomycin products.
Vancomycin prevents peptidoglycan synthesis by two separate mechanisms. One mechanism prevents N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) from linking together forming the peptidoglycan backbone. The second mechanism prevents crosslinking between amino acid residues in the peptidoglycan chain.
Vancomycin inhibits growth of many Gram positive bacteria including the antibiotic resistant superbug, MRSA (Methicillin resistant Staphylococcus aureus). Vancomcyin is effective for treating MRSA infections because it inhibits cell wall synthesis through a different mechanism than β-lactam antibiotics.
Vancomycin HCl is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Vancomycin is commonly used in selective media for superbug vancomycin resistant enterococcus (VRE) detection. Representative MIC values include:
- Methicillin resistant Staphylococcus aureus (MRSA) 0.25 µg/mL - 2 µg/mL
- Clostridium difficile 0.06 µg/mL - 4 µg/mL
- For a complete list of vancomycin HCl MIC values, click here.
Vancomycin can be used as a selective agent in several types of isolation media:
Brucella medium - Brucella selective supplement
Columbia Blood Agar - Campylobacter selective supplement (Skirrow)
Columbia Blood Agar - Campylobacterselective supplement (Blaser-Wang)
Wilkins-Chalgren Anaerobe Agar - Isolation of Gram-negative anaerobes
Legionella CYE Agar - Legionella MWY Selective Supplement
Columbia Blood Agar - Helicobacter pylori Selective Supplement (Dent)
Legionella CYE Agar - Legionella GVPC Selective Supplement
Campylobacter Agar - Campylobacter Selective Supplement (Karmali)
Bolton Broth - Bolton Broth Selective Supplement
VRE Medium - VRE Selective Supplement
mTSB - VCC Selective Supplement
Campylobacter Agar Base - Modified Karmali Selective Supplement
Bolton Broth - Modified Bolton Broth Selective Supplement
Brucella Medium Base - Modified Brucella Selective Supplement
Legionella CYE Agar - Legionella GVPN Selective Supplement
Plant Biology Applications
Vancomycin is often used in Agrobacterium mediated transformations to control bacterial growth and shows a synergistic effect when used in combination with cefotaxime. Vancomycin does not appear to have any negative effects on plants; however, a study by Silva and Fukai (2001) observed a lower transformation efficiency when >500 µg/ml was used.
For in vitro research use only. Not suitable for human or animal consumption.
Courvalin, Patrice. "Vancomycin Resistance in Gram-Positive Cocci." Oxford Journals(2006): 25-34. Clinical Infectious Diseaes. Web. 21 Aug. 2012.
Pollock, H.M., Holt J., and Murray C., Comparison of susceptibilities of anaerobic bacteria to cefemenoxime, ceftriaxone and other antimicrobial compounds, Antimicrob. Agents Chemother., Vol. 23, pp.780-783, 1983
Silva J.A. and Fukai S., The impact of carbenicillin, cefotaxime and vancomycin on chrysanthemum and tobacco TCL morphogenesis and Agrobacterium growth, J. Appl. Hort., Vol. 3(1), pp. 3-12, 2001.
Joshi, S., Ray, P., Manchanda, V., Bajaj, J., Chitnis, D. S., Gautam, V., &...Balaji, V. (2013). Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence & susceptibility pattern. Indian Journal Of Medical Research, 137(2), 363-369