Antimicrobials > Anti-bacterials/Gene Selectors > Others > Puromycin Dihydrochloride
Puromycin Dihydrochloride, Solution
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Puromycin is an aminoglycoside antibiotic that inhibits the synthesis of protein by binding to the ribosome and disrupting translocation of tRNA during translation in non-resistant cells. Puromycin is soluble in water and is often used as a selective agent in molecular biology research. The concentration of our Puromycin solution is 10mg/ml. 1ml is usally added to 1L of cell culture media.

Mechanism:Puromycin enters the A site and transfers to the growing chain, causing premature chain release. The exact mechanism of action is unknown at this time, but, the 3' position contains an amide linkage instead of the normal ester linkage of tRNA, the amide bond makes the molecule much more resistant to hydrolysis and thus stops the ribosome.
Molecular Weight:544.43
CAS NO.:58-58-2
Molecular Formula:

C22H29N7O5.2HCl

Melting point:174 °C
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