Norfloxacin is a first generation synthetic fluoroquinolone antibiotic and is sparingly soluble in aqueous solution at 1mg/mL.
Fluoroquinolone antibiotics target bacterial DNA gyrase, an enzyme which reduces DNA strain during replication. Because DNA gyrase is required during DNA replication, subsequent DNA synthesis and ultimately cell division is inhibited.
Norfloxacin is a broad spectrum antibiotic commonly used to target bacteria responsible for urinary tract infections.
Norfloxacin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram positive and gram negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. Representative MIC values include:
- Escherichia coli 0.25 µg/mL – 10 µg/mL
- For a complete list of norfloxacin MIC values, click here.
Norfloxacin can be used as a selective agent in several types of isolation media:
Clostridium difficile Agar - CDMN Selective Supplement
Wolfson, John S., and David C. Hooper. "The Fluoroquinolones: Structures, Mechanisms of Action and Resistance, and Spectra of Activity in Vitro." American Society for Microbiology 4th ser. 28 (1985): 581-86.