Nitrocefin is a cephalosporin with chromogenic properties and is routinely used to detect beta-lactamase enzymes produced by beta-lactam resistant bacteria. Nitrocefin is soluble in DMSO and is commonly used at a 1.0 mg/mL concentration.
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.
Essentially all beta-lactamase enzymes hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the beta-lactam ring of nitrocefin. Macroscopic detection of this process is made possible because a ultraviolet absorption shift from intact versus hydrolyzed nitrocefin occurs within the visible light spectrum (~380 nm to ~500 nm, or yellow to red).
For in vitro research use only. Not suitable for human or animal consumption. For MSDSs not available online, please email a request to email@example.com
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Parr, T. R., Jr. "Simple Screening Method for Beta-lactamase-positive and -negative Ampicillin-resistant Haemophilus Influenzae Isolates." Journal of Clinical
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