Nitrocefin is a cephalosporin with chromogenic properties and is routinely used to detect beta-lactamase enzymes produced by beta-lactam resistant bacteria. Nitrocefin is soluble in DMSO and is commonly used at a 1.0 mg/mL concentration.
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for specific questions.
Essentially all beta-lactamase enzymes hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the beta-lactam ring of nitrocefin. Macroscopic detection of this process is made possible because a ultraviolet absorption shift from intact versus hydrolyzed nitrocefin occurs within the visible light spectrum (~380 nm to ~500 nm, or yellow to red).
For in vitro research use only. Not suitable for human or animal consumption.
O'Callaghan, Cynthia H. et. al. "Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate." Antimicrobial Agents and
Chemotherapy 1.4 (1972): 283-88. Ncbi.gov Web. 10 Oct. 2012.
Parr, T. R., Jr. "Simple Screening Method for Beta-lactamase-positive and -negative Ampicillin-resistant Haemophilus Influenzae Isolates." Journal of Clinical
Microbiology 20.1 (1984): 131-32. Ncbi.gov. Web. 10 Oct. 2012.